|
Three Root Biomass and Bacterial Count Trials
using Humate and Viresco
Introduction
Between July and September 1998, London University
carried out a series of tests at 4 golf courses in the south of
England. The 72 greens at the 4 courses along with one practice
green were used as the test areas. The trials looked at changes
in bacterial counts and root biomass.
During the football season 1999/2000, a trial was
conducted at The Dell, the ground of Southampton FC using Humates
and Viresco to see their effect on root biomass and bacterial counts.
During the summer 2000, a trial was carried out using
a foliar Viresco™ product on grass leaf. This test looked
at populations of bacteria on the leaf and their longevity.
Golf Course Trial
Two separate trials were carried out. In one trial,
the 72 greens were all treated with only Viresco™ Foliar.
Because in every case the whole of the green was treated, the collars
around the greens were used as control.
This trial started at the beginning of July and continued
to the end of September, a period of about 3 months. Microbial counts
were made prior to treatment in early July and at the end of September
1998. Root biomass checks were made at the same times.
In the other trial, a practice green at one of the
courses was used. Twenty one-metre squares were measured out. Ten
areas were used for the test and the other ten used as control.
For the test areas medium grade granular Humate, Viresco™
Soluble and Viresco™ Foliar were used. The Humate was put
on as a pre-treatment, followed by an application of Viresco™
Soluble as an inoculant with monthly applications of Viresco™
Foliar. The trial started in late July and finished in late September,
ie a period of only 2 months. As in the main trial, bacterial counts
and root biomass measurements were made immediately prior to the
trial and again at the end of September.
Bacterial counts are expressed in millions per gram
of soil. Root biomass is expressed in grams and is an average of
3 x 2½" cores.
In the trial on the 72 greens using only Viresco™
Foliar, there was a statistically significant increase in the microbial
counts and in the root biomass. Bacterial counts went up on average
by about 27.5% whilst in the control plots it went down by about
5%.
The root biomass increased on average in the test
plots by about 15% whereas in the control plots it went down by
less than 1%, ie in effect, no change.
| 
|
|
| |
In the trial on the practice green
where Granular Humate, Viresco™ Soluble and Viresco™
Foliar were used over a shorter period of time (2 months instead
of 3 months).
The bacterial count went up on average by 98.8%,
ie from 1.67 million per gram to 3.32 million per gram, in
the test plots whereas in the control plots it went down by
8.7%. The root biomass in the test plots increased on average
by 29.4%, ie from 4.89g to 6.33g, whereas in the control plots
there was no change
The first bar chart shows the bacterial counts
on the 10 untreated plots at the beginning and at the end
of the two months trial period. The second bar chart shows
the counts on the treated plots before and after the trial.
|
| 
|
|
| |
| The third and fourth bar charts
relate to the root biomass test over the two month trial period.
One chart shows the changes in root mass in the untreated
plots and the other shows the changes in the treated plots. |
Southampton FC
This trial started in October 1999 and lasted for
about 10 months. Three separate trials were conducted. The first
looked at the application of Humate granular and Humate liquid on
their own. The second involved Viresco™ micro-organism products
on their own. The third trial considered both the Humate and the
Viresco™ microbial products. All three programmes increased
bacterial counts and root biomass. However, the effect of the combined
Humate and Viresco™ programme was much more significant than
either the Humate or Viresco™ programme on its own.
| 
|
|
| |
| Bacterial counts more than
doubled in the first month and root biomass more than doubled
within three months compared with the control plots. These improvements
were maintained throughout the period of the study. These results
are shown on the adjacent two graphs It
is worth mentioning that the root biomass checks were
done on soil cores 1" in diameter and 2.5 inches deep.
The actual root biomass with the Humate and Viresco™
programmes would most likely be deeper than this and would,
therefore, indicate an even larger increase. |
This study demonstrates once again the significant
improvements in microbial activity and plant root biomass that are
typical of the joint Humate and Viresco™ programme.
The resulting microbial population is not only more
active, it is more diverse with significantly increased competition
in the plant rhizosphere. The result is a more efficient, balanced
soil environment. Furthermore, the turf is healthier and more resistant
to pathogens and other stresses and require significantly reduced
nutrient and chemical inputs.
Bacterial Counts
on Leaf - Longevity of Bacteria
There is a commonly held, but incorrect belief, that
applying bacteria to leaf is pointless as they quickly die because
of the damaging effect of ultra violet radiation in sunlight. There
is no question that UV does kill bacteria. However, our foliar Viresco
products are formulated so as to give considerable protection from
UV light to the bacteria they contain.
There is available a piece of equipment that golf
greenkeepers use daily to "brew up" microbial mixes for
immediate application to golf greens. This equipment is not needed
as our foliar products can raise dramatically and keep at a high
level for many days the bacterial counts on leaf. During the summer
of 2000, London University carried out a test on turf grass to look
at this question.
The product used for the trial is a 50% constituent
of Viresco™ Foliar. Prior to application to the grass leaf,
bacterial counts were done on ten replicates. Further counts, expressed
as millions per sq. cm., were done on a regular basis and the results
were as follows:
| Prior to the experiment |
5.60 million |
| After 1 day |
12.44 million |
| After 7 days |
13.52 million |
| After 14 days |
8.99 million |
| After 21 days |
5.66 million |
| After 28 days |
6.73 million |
| After 35 days |
4.59 million |
| After 41 days |
4.34 million |
|
|
It can be readily seen that the bacterial count immediately
increased after application and whilst there was then a falling
back in numbers, reasonably high counts were maintained for over
14 days.
It is worth pointing out that no other application of either Humate
or Viresco™ was made to the soil in which the grass was growing.
We believe that if a full Humate/Viresco™ programme had been
in place then the bacterial counts would have been larger and lasted
longer at the higher levels.
Comment
Very impressive increases in bacterial counts can
be made and maintained when Humate and Viresco™ products are
used on greens and sports fields. However, the bacterial counts
on golf greens vary considerably according to the pH of the soil
and the type of green.
Microbes have many functions and high counts are important
in regard to good turf management. These functions include the breakdown
of organic compounds including lignin and cellulose, the formation
of humus, the stabilisation of soil, nutrient recycling, fixing
of nitrogen from air and disease suppression.
The trials showed that an extremely large increase
in root biomass can be achieved in a relatively short period when
the Humate and Viresco™ products are used. Greenkeepers and
groundsmen are constantly striving to increase the root biomass
on their turf. Our Humate and Viresco™ programmes can give
them, and others, what they are seeking!

|